6BYX

Complex structure of LOR107 mutant (R259N) with tetrasaccharide substrate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Short ulvan lyase	polymer	506	57517.1	2	UniProt (A0A109PTH9) Pfam (PF15892) In PDB	Alteromonas sp. LOR
2	C, D	4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-3-O-sulfo-alpha-L-rhamnopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-4)-3-O-sulfo-alpha-L-rhamnopyranose	branched		804.7	2	In PDB GlyTouCan (G96014EV)
3	A, B	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
4	A, B	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
5	B, A	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
6		water	water		18.0	465	Chemie (HOH)

Sequence modifications

A, B: 26 - 522 (UniProt: A0A109PTH9)

PDB	External Database	Details
Met 25	-	initiating methionine
Asn 259	Arg 265	engineered mutation
Leu 523	-	expression tag
Glu 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag
His 529	-	expression tag
His 530	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	115034.3
Branched	Number of molecules	2
	Total formula weight	1609.3
Non-Polymers*	Number of molecules	23
	Total formula weight	1949.8
All*	Total formula weight	118593.4

*Water molecules are not included.