6BYG

Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-mannosidase	polymer	860	96830.0	2	UniProt (Q8PI23)	Xanthomonas axonopodis pv. citri (strain 306)
2	C, D (A, B)	beta-D-mannopyranose	non-polymer		180.2	2	Chemie (BMA)
3	E, F (A, B)	water	water		18.0	1224	Chemie (HOH)

Sequence modifications

A, B: 32 - 888 (UniProt: Q8PI23)

PDB	External Database	Details
Ser 29	-	expression tag
His 30	-	expression tag
Met 31	-	expression tag
Ala 575	Glu 575	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	193660.0
Non-Polymers*	Number of molecules	2
	Total formula weight	360.3
All*	Total formula weight	194020.3

*Water molecules are not included.