6BYG
Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.445, 116.048, 104.936 |
| Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
| Resolution | 43.147 - 1.999 |
| R-factor | 0.1729 |
| Rwork | 0.171 |
| R-free | 0.21540 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.236 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.147 | 5.950 | 2.270 |
| High resolution limit [Å] | 1.990 | 4.230 | 2.120 |
| Number of reflections | 121038 | ||
| <I/σ(I)> | 10.57 | ||
| Completeness [%] | 95.1 | ||
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 6000 Sodium acetate Magnesium chloride |
