6BXK

Crystal structure of Pyrococcus horikoshii Dph2 with 4Fe-4S cluster and MTA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	2-(3-amino-3-carboxypropyl)histidine synthase	polymer	378	42538.3	2	UniProt (O58832)	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	Diphthamide biosynthesis protein Dph2,S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase
2	C, F (A, B)	5'-DEOXY-5'-METHYLTHIOADENOSINE	non-polymer		297.3	2	Chemie (MTA)
3	D, G (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E, H (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
5	I, J (A, B)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 1 - 342 (UniProt: O58832)

PDB	External Database	Details
Met -35	-	initiating methionine
Gly -34	-	expression tag
Ser -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
Asp -26	-	expression tag
Ile -25	-	expression tag
Thr -24	-	expression tag
Ser -23	-	expression tag
Leu -22	-	expression tag
Tyr -21	-	expression tag
Lys -20	-	expression tag
Lys -19	-	expression tag
Ala -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ala -15	-	expression tag
Ala -14	-	expression tag
Ala -13	-	expression tag
Val -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	85076.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1490.1
All*	Total formula weight	86566.7

*Water molecules are not included.