6BUZ
Cryo-EM structure of CENP-A nucleosome in complex with kinetochore protein CENP-N
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Histone H3-like centromeric protein A | polymer | 160 | 18195.0 | 2 | UniProt (P49450) Pfam (PF00125) In PDB | Homo sapiens (Human) | Centromere autoantigen A,Centromere protein A,CENP-A |
2 | B, F | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62805) Pfam (PF15511) In PDB | Homo sapiens (Human) | |
3 | C, G | Histone H2A | polymer | 130 | 14165.6 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB | Homo sapiens (Human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
4 | D, H | Histone H2B | polymer | 126 | 13935.2 | 2 | UniProt (P06899) Pfam (PF00125) In PDB | Homo sapiens (Human) | Histone H2B.1,Histone H2B.r,H2B/r |
5 | I | DNA (147-MER) | polymer | 147 | 45138.8 | 1 | synthetic construct | ||
6 | J | DNA (147-MER) | polymer | 147 | 45610.0 | 1 | synthetic construct | ||
7 | N | Maltose-binding periplasmic protein, Centromere protein N chimera | polymer | 668 | 75607.8 | 1 | UniProt (P0AEY0) UniProt (Q96H22) Pfam (PF01547) Pfam (PF05238) UniProt (by SIFTS) (P0AEX9) In PDB | Escherichia coli | MBP, MMBP, Maltodextrin-binding protein, CENP-N, Interphase centromere complex protein 32 |
Sequence modifications
A, E: 1 - 140 (UniProt: P49450)
N: -370 - -2 (UniProt: P0AEY0)
N: 1 - 286 (UniProt: Q96H22)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -381 | - | expression tag |
Lys -380 | - | expression tag |
Glu -379 | - | expression tag |
His -378 | - | expression tag |
His -377 | - | expression tag |
His -376 | - | expression tag |
His -375 | - | expression tag |
His -374 | - | expression tag |
His -373 | - | expression tag |
His -372 | - | expression tag |
His -371 | - | expression tag |
Ala -11 | Glu 385 | engineered mutation |
Ala -8 | Lys 388 | engineered mutation |
Ala -7 | Asp 389 | engineered mutation |
Asn -3 | Arg 393 | engineered mutation |
Ala -2 | Ile 394 | engineered mutation |
Ala -1 | - | linker |
Ala 0 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 11 |
Total formula weight | 281737.0 | |
All* | Total formula weight | 281737.0 |