6BUZ

Cryo-EM structure of CENP-A nucleosome in complex with kinetochore protein CENP-N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3-like centromeric protein A	polymer	160	18195.0	2	UniProt (P49450) Pfam (PF00125)	Homo sapiens (Human)	Centromere autoantigen A,Centromere protein A,CENP-A
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, G (C, G)	Histone H2A	polymer	130	14165.6	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B	polymer	126	13935.2	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I (I)	DNA (147-MER)	polymer	147	45138.8	1		synthetic construct
6	J (J)	DNA (147-MER)	polymer	147	45610.0	1		synthetic construct
7	K (N)	Maltose-binding periplasmic protein, Centromere protein N chimera	polymer	668	75607.8	1	UniProt (P0AEY0) UniProt (Q96H22) Pfam (PF01547) Pfam (PF05238) UniProt (by SIFTS) (P0AEX9)	Escherichia coli	MBP, MMBP, Maltodextrin-binding protein, CENP-N, Interphase centromere complex protein 32

Sequence modifications

A, E: 1 - 140 (UniProt: P49450)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

N: -370 - -2 (UniProt: P0AEY0)

PDB	External Database	Details
Met -381	-	expression tag
Lys -380	-	expression tag
Glu -379	-	expression tag
His -378	-	expression tag
His -377	-	expression tag
His -376	-	expression tag
His -375	-	expression tag
His -374	-	expression tag
His -373	-	expression tag
His -372	-	expression tag
His -371	-	expression tag
Ala -11	Glu 385	engineered mutation
Ala -8	Lys 388	engineered mutation
Ala -7	Asp 389	engineered mutation
Asn -3	Arg 393	engineered mutation
Ala -2	Ile 394	engineered mutation
Ala -1	-	linker
Ala 0	-	linker

N: 1 - 286 (UniProt: Q96H22)

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	281737.0
All*	Total formula weight	281737.0

*Water molecules are not included.