6BUQ
Crystal structures of cyanuric acid hydrolase from Moorella thermoacetica complexed with barbituric acid
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Cyanuric acid amidohydrolase | polymer | 363 | 38484.7 | 4 | UniProt (Q2RGM7) Pfam (PF09663) In PDB | Moorella thermoacetica (strain ATCC 39073 / JCM 9320) | CAH, Barbiturase |
2 | A | MALONATE ION | non-polymer | 102.0 | 1 | Chemie (MLI) | |||
3 | A, B, C, D | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
4 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
5 | A, B, C, D | 1,3-PROPANDIOL | non-polymer | 76.1 | 9 | Chemie (PDO) | |||
6 | A, B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
7 | B, C, D | BARBITURIC ACID | non-polymer | 128.1 | 3 | Chemie (BR8) | |||
8 | D | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
9 | water | water | 18.0 | 797 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 367 (UniProt: Q2RGM7)
PDB | External Database | Details |
---|---|---|
His 0 | - | expression tag |
Ala 103 | Gln 103 | engineered mutation |
Ala 104 | Glu 104 | engineered mutation |
Ala 107 | Lys 107 | engineered mutation |
Ile 279 | Leu 279 | engineered mutation |
Arg 280 | Lys 280 | engineered mutation |
Ser 281 | Phe 281 | engineered mutation |
- | Cys 283 | deletion |
- | Cys 284 | deletion |
- | Pro 285 | deletion |
- | Pro 286 | deletion |
- | Ala 287 | deletion |
Asp 283 | Glu 288 | engineered mutation |
Met 290 | Leu 290 | engineered mutation |
Asp 291 | Ala 291 | engineered mutation |
Arg 292 | Lys 292 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 153938.6 | |
Non-Polymers* | Number of molecules | 21 |
Total formula weight | 1531.5 | |
All* | Total formula weight | 155470.1 |