6BSR

Crystal structure of penicillin-binding protein 4 (PBP4) from Enterococcus faecalis in the benzylpenicillin bound form.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	PBP4 protein	polymer	666	72965.6	1	UniProt (Q9K3C9) Pfam (PF05223) Pfam (PF03717) Pfam (PF00905) In PDB	Enterococcus faecalis (Streptococcus faecalis)	Penicillin-binding protein,Penicillin-binding transpeptidase domain-containing protein
2	A	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	A	OPEN FORM - PENICILLIN G	non-polymer		336.4	1	Chemie (PNM)		Penicillin-binding protein
6		water	water		18.0	391	Chemie (HOH)

Sequence modifications

A: 36 - 680 (UniProt: Q9K3C9)

PDB	External Database	Details
Met 15	-	initiating methionine
Gly 16	-	expression tag
Ser 17	-	expression tag
Asp 18	-	expression tag
Lys 19	-	expression tag
Ile 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
Glu 27	-	expression tag
Asn 28	-	expression tag
Leu 29	-	expression tag
Tyr 30	-	expression tag
Phe 31	-	expression tag
Gln 32	-	expression tag
Gly 33	-	expression tag
His 34	-	expression tag
Met 35	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	72965.6
Non-Polymers*	Number of molecules	9
	Total formula weight	973.9
All*	Total formula weight	73939.5

*Water molecules are not included.