6BSR
Crystal structure of penicillin-binding protein 4 (PBP4) from Enterococcus faecalis in the benzylpenicillin bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-09-28 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.34 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.306, 85.469, 83.685 |
| Unit cell angles | 90.00, 109.46, 90.00 |
Refinement procedure
| Resolution | 17.650 - 2.340 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.478 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.700 | 2.380 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Number of reflections | 34537 | |
| <I/σ(I)> | 28.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 40 mM potassium phosphate monobasic, 18% PEG8000, 22% Glycerol |
