6BM9

Directed evolutionary changes in MBL super family - VIM-2 Round 10

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Metallo-beta-lactamase	polymer	243	25881.9	4	UniProt (Q5U7L7) Pfam (PF00753) In PDB	Escherichia coli	VIM2R10
2	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	B, C	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4		water	water		18.0	195	Chemie (HOH)

Sequence modifications

A, B, C, D: 27 - 266 (UniProt: Q5U7L7)

PDB	External Database	Details
Gly 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Arg 31	Gly 31	conflict
Ala 36	Val 36	conflict
Asp 41	Val 41	conflict
Ala 59	Thr 59	conflict
Pro 61	Ser 61	conflict
Leu 62	Phe 62	conflict
Ala 66	Val 66	conflict
Lys 144	Glu 144	conflict
Thr 148	Asn 148	conflict
Pro 181	Thr 181	conflict
Ile 185	Val 185	conflict
Arg 192	Ser 192	conflict
Ala 213	Asp 213	conflict
Ser 253	Thr 253	conflict
Asp 254	Asn 254	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	103527.5
Non-Polymers*	Number of molecules	6
	Total formula weight	445.8
All*	Total formula weight	103973.3

*Water molecules are not included.