6BHJ

Structure of HIV-1 Reverse Transcriptase Bound to a 38-mer Hairpin Template-Primer RNA-DNA Aptamer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	HIV-1 REVERSE TRANSCRIPTASE P66 subunit	polymer	557	64135.4	2	UniProt (A0A076Q3N8) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) UniProt (by SIFTS) (P03366) In PDB	Human immunodeficiency virus 1
2	B, D	HIV-1 REVERSE TRANSCRIPTASE P51 subunit	polymer	444	51928.6	2	UniProt (A0A076Q3N8) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) UniProt (by SIFTS) (P03366) In PDB	Human immunodeficiency virus 1
3	E, F	38-MER RNA-DNA Aptamer	polymer	38	11896.3	2		synthetic construct
4	G, H	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
5	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	B, C, D	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
7	C	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
8		water	water		18.0	111	Chemie (HOH)

Sequence modifications

A, C: -1 - 555 (UniProt: A0A076Q3N8)

PDB	External Database	Details
Lys 172	Arg 339	variant
Ser 280	Cys 447	engineered mutation
Asn 498	Asp 665	engineered mutation

B, D: 1 - 428 (UniProt: A0A076Q3N8)

PDB	External Database	Details
Met -15	-	initiating methionine
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Lys 172	Arg 339	variant
Ser 280	Cys 447	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	255920.6
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	9
	Total formula weight	697.2
All*	Total formula weight	257302.4

*Water molecules are not included.