6BCU

Cryo-EM structure of the activated RHEB-mTORC1 refined to 3.4 angstrom

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, B)	Serine/threonine-protein kinase mTOR,Serine/threonine-protein kinase mTOR	polymer	2549	287399.1	2	UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260)	Homo sapiens (Human)	FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin
2	B, G (D, E)	Target of rapamycin complex subunit LST8	polymer	326	35910.1	2	UniProt (Q9BVC4) Pfam (PF00400)	Homo sapiens (Human)	TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8
3	C, H (W, Y)	Regulatory-associated protein of mTOR,Regulatory-associated protein of mTOR	polymer	1343	150197.0	2	UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400)	Homo sapiens (Human)	Raptor,p150 target of rapamycin (TOR)-scaffold protein,Raptor,p150 target of rapamycin (TOR)-scaffold protein
4	D, I (X, Z)	Eukaryotic translation initiation factor 4E-binding protein 1	polymer	122	12951.3	2	UniProt (Q13541) Pfam (PF05456)	Homo sapiens (Human)	eIF4E-binding protein 1,Phosphorylated heat- and acid-stable protein regulated by insulin 1,PHAS-I
5	E, J (S, R)	GTP-binding protein Rheb	polymer	188	20877.8	2	UniProt (Q15382) Pfam (PF00071)	Homo sapiens (Human)	Ras homolog enriched in brain
6	K, P (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
7	L, M, O, Q, R... (A, S, B, R)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
8	N, S (S, R)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

A, B: 1 - 579 (PDB: 6BCU)
A, B: 579 - 2549 (UniProt: P42345)
W, Y: 2 - 661 (UniProt: Q8N122)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

W, Y: 662 - 1335 (UniProt: Q8N122)
X, Z: 1 - 118 (UniProt: Q13541)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

S, R: 1 - 184 (UniProt: Q15382)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	1014670.8
Non-Polymers*	Number of molecules	10
	Total formula weight	2206.6
All*	Total formula weight	1016877.3

*Water molecules are not included.