6BCU
Cryo-EM structure of the activated RHEB-mTORC1 refined to 3.4 angstrom
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F (A, B) | Serine/threonine-protein kinase mTOR,Serine/threonine-protein kinase mTOR | polymer | 2549 | 287399.1 | 2 | UniProt (P42345) Pfam (PF11865) Pfam (PF02259) Pfam (PF23593) Pfam (PF08771) Pfam (PF00454) Pfam (PF02260) | Homo sapiens (Human) | FK506-binding protein 12-rapamycin complex-associated protein 1,FKBP12-rapamycin complex-associated protein,Mammalian target of rapamycin |
| 2 | B, G (D, E) | Target of rapamycin complex subunit LST8 | polymer | 326 | 35910.1 | 2 | UniProt (Q9BVC4) Pfam (PF00400) | Homo sapiens (Human) | TORC subunit LST8,G protein beta subunit-like,Protein GbetaL,Mammalian lethal with SEC13 protein 8,mLST8 |
| 3 | C, H (W, Y) | Regulatory-associated protein of mTOR,Regulatory-associated protein of mTOR | polymer | 1343 | 150197.0 | 2 | UniProt (Q8N122) Pfam (PF14538) Pfam (PF02985) Pfam (PF00400) | Homo sapiens (Human) | Raptor,p150 target of rapamycin (TOR)-scaffold protein,Raptor,p150 target of rapamycin (TOR)-scaffold protein |
| 4 | D, I (X, Z) | Eukaryotic translation initiation factor 4E-binding protein 1 | polymer | 122 | 12951.3 | 2 | UniProt (Q13541) Pfam (PF05456) | Homo sapiens (Human) | eIF4E-binding protein 1,Phosphorylated heat- and acid-stable protein regulated by insulin 1,PHAS-I |
| 5 | E, J (S, R) | GTP-binding protein Rheb | polymer | 188 | 20877.8 | 2 | UniProt (Q15382) Pfam (PF00071) | Homo sapiens (Human) | Ras homolog enriched in brain |
| 6 | K, P (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 7 | L, M, O, Q, R... (A, S, B, R) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 8 | N, S (S, R) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
A, B: 1 - 579 (PDB: 6BCU)
A, B: 579 - 2549 (UniProt: P42345)
W, Y: 2 - 661 (UniProt: Q8N122)
W, Y: 662 - 1335 (UniProt: Q8N122)
X, Z: 1 - 118 (UniProt: Q13541)
S, R: 1 - 184 (UniProt: Q15382)
A, B: 579 - 2549 (UniProt: P42345)
W, Y: 2 - 661 (UniProt: Q8N122)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Lys 1 | - | expression tag |
X, Z: 1 - 118 (UniProt: Q13541)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Arg 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 1014670.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2206.6 | |
| All* | Total formula weight | 1016877.3 |
