6BCA

A Complex between PH Domain of LbcRhoGEF (AKAP-Lbc) and Activated RhoA Bound to a GTP Analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (F, C)	Transforming protein RhoA	polymer	185	20865.9	2	UniProt (P61586) Pfam (PF00071)	Homo sapiens (Human)	Rho cDNA clone 12,h12
2	B, C (A, B)	A-kinase anchor protein 13	polymer	151	17491.3	2	UniProt (Q12802) Pfam (PF00169)	Homo sapiens (Human)	AKAP-13,AKAP-Lbc,Breast cancer nuclear receptor-binding auxiliary protein,Guanine nucleotide exchange factor Lbc,Human thyroid-anchoring protein 31,Lymphoid blast crisis oncogene,LBC oncogene,Non-oncogenic Rho GTPase-specific GTP exchange factor,Protein kinase A-anchoring protein 13,PRKA13,p47
3	E, G (F, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, H (F, C)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	2	Chemie (GSP)
5	I, J, K, L (F, A, B, C)	water	water		18.0	487	Chemie (HOH)

Sequence modifications

F, C: 1 - 181 (UniProt: P61586)

PDB	External Database	Details
Gly -3	-	expression tag
Ile -2	-	expression tag
Leu -1	-	expression tag
Asp 0	-	expression tag

A, B: 2193 - 2333 (UniProt: Q12802)

PDB	External Database	Details
Gly 2189	-	expression tag
Ile 2190	-	expression tag
Leu 2191	-	expression tag
Asp 2192	-	expression tag
His 2334	-	expression tag
His 2335	-	expression tag
His 2336	-	expression tag
His 2337	-	expression tag
His 2338	-	expression tag
His 2339	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76714.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1127.1
All*	Total formula weight	77841.6

*Water molecules are not included.