6BC1

A Complex between PH Domain of p190RhoGEF and Activated Rac1 Bound to a GTP Analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Ras-related C3 botulinum toxin substrate 1	polymer	183	20505.6	2	UniProt (P63000) Pfam (PF00071)	Homo sapiens (Human)	Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1
2	B, D (C, D)	Rho guanine nucleotide exchange factor 28	polymer	150	17533.2	2	UniProt (Q8N1W1) Pfam (PF17838)	Homo sapiens (Human)	190 kDa guanine nucleotide exchange factor,p190RhoGEF,Rho guanine nucleotide exchange factor
3	E, G (A, B)	5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE	non-polymer		539.2	2	Chemie (GSP)
4	F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	I, J, K, L (A, C, B, D)	water	water		18.0	113	Chemie (HOH)

Sequence modifications

A, B: 2 - 177 (UniProt: P63000)

PDB	External Database	Details
Pro 1	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag

C, D: 1049 - 1194 (UniProt: Q8N1W1)

PDB	External Database	Details
Gly 1045	-	expression tag
Ile 1046	-	expression tag
Leu 1047	-	expression tag
Asp 1048	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	76077.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1127.1
All*	Total formula weight	77204.6

*Water molecules are not included.