6BBN

Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)	Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
2	B, D (B, D)	Tubulin beta-2B chain	polymer	445	49999.9	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (Bovine)
3	E (P)	DARPin	polymer	168	17911.2	1		Escherichia coli
4	F (E)	Kinesin-like protein KIF2A	polymer	420	47918.9	1	UniProt (O00139) Pfam (PF00225)	Homo sapiens (Human)	Kinesin-2, hK2
5	G, K (A, C)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	H, J, L, P (A, B, C, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	I, M (B, D)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
8	N (D)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
9	O (E)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)

Sequence modifications

E: 153 - 553 (UniProt: O00139)

PDB	External Database	Details
Met 134	-	expression tag
Gly 135	-	expression tag
Ser 136	-	expression tag
Ser 137	-	expression tag
His 138	-	expression tag
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
Ser 144	-	expression tag
Ser 145	-	expression tag
Gly 146	-	expression tag
Leu 147	-	expression tag
Val 148	-	expression tag
Pro 149	-	expression tag
Arg 150	-	expression tag
Gly 151	-	expression tag
Ser 152	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	266238.8
Non-Polymers*	Number of molecules	10
	Total formula weight	2631.1
All*	Total formula weight	268870.0

*Water molecules are not included.