6BBN

Crystal structure of a curved tubulin complex induced by the kinesin-13 Kif2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	145.052, 82.188, 147.658
Unit cell angles	90.00, 109.33, 90.00

Refinement procedure

Resolution	49.217 - 3.514
R-factor	0.2391
Rwork	0.236
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4LNU & 2GRY
RMSD bond length	0.004
RMSD bond angle	0.689
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.580
High resolution limit [Å]	3.460	7.450	3.460
Rmerge	0.212	0.041	2.235
Rmeas	0.249	0.049	2.685
Rpim	0.131	0.025	1.469
Total number of observations	142449
Number of reflections	40041	4151	3869
<I/σ(I)>	4.5
Completeness [%]	99.3	99.7	96.9
Redundancy	3.6	3.6	3.1
CC(1/2)		0.998	0.212

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	8% PEG8000, 6% ethylene glycol, 10 mM DDT, 100 mM HEPES, pH 7.5