6B70

Cryo-EM structure of human insulin degrading enzyme in complex with FAB H11-E heavy chain, FAB H11-E light chain and insulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Insulin-degrading enzyme	polymer	966	111866.5	2	UniProt (P14735) Pfam (PF00675) Pfam (PF05193) Pfam (PF16187) In PDB	Homo sapiens (Human)	Abeta-degrading protease,Insulin protease,Insulinase,Insulysin
2	C, E	FAB H11-E heavy chain	polymer	218	23057.7	2	UniProt (P0DOX5) Pfam (PF07686) Pfam (PF07654) In PDB	Mus musculus (Mouse)
3	D, F	FAB H11-E light chain	polymer	211	23087.6	2	UniProt (Q6GMX0) Pfam (PF07686) Pfam (PF07654) In PDB	Mus musculus (Mouse)
4	a, c	Insulin	polymer	110	11989.9	2	UniProt (P01308) Pfam (PF00049) In PDB	Homo sapiens (Human)

Sequence modifications

A, B: 46 - 1011 (UniProt: P14735)

PDB	External Database	Details
Leu 110	Cys 110	conflict
Ser 171	Cys 171	conflict
Ala 178	Cys 178	conflict
Val 257	Cys 257	conflict
Leu 414	Cys 414	conflict
Asn 573	Cys 573	conflict
Ser 590	Cys 590	conflict
Ser 789	Cys 789	conflict
Ala 812	Cys 812	conflict
Ala 819	Cys 819	conflict
Ser 904	Cys 904	conflict

C, E: 4 - 126 (PDB: 6B70)
C, E: 127 - 221 (UniProt: P0DOX5)
D, F: 2 - 105 (PDB: 6B70)
D, F: 106 - 212 (UniProt: Q6GMX0)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	340003.4
All*	Total formula weight	340003.4

*Water molecules are not included.