6B3S
Crystal structure of the Fab fragment of necitumumab (Fab11F8) in complex with domain III from a cetuximab resistant variant of EGFR (sEGFRd3-S468R)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, G, J (A, B, E, I) | Epidermal growth factor receptor | polymer | 214 | 23856.2 | 4 | UniProt (P00533) Pfam (PF01030) | Homo sapiens (Human) | Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1 |
| 2 | B, E, H, K (H, C, F, J) | Necitumumab Fab Heavy chain | polymer | 222 | 23547.3 | 4 | Homo sapiens (Human) | ||
| 3 | C, F, I, L (L, D, G, K) | Necitumumab Fab Light chain | polymer | 213 | 23053.6 | 4 | Homo sapiens (Human) | ||
| 4 | M, O, Q, S, T (M, O, Q, S, T) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 5 | In PDB GlyTouCan (G42666HT) | |||
| 5 | N (N) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 6 | P, R (P, R) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 7 | AA, U, V, W, X... (I, A, B, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 7 | Chemie (NAG) | |||
| 8 | BA, CA, DA, EA, FA... (A, H, L, B, C...) | water | water | 18.0 | 69 | Chemie (HOH) |
Sequence modifications
A, B, E, I: 311 - 514 (UniProt: P00533)
| PDB | External Database | Details |
|---|---|---|
| Leu 307 | - | expression tag |
| Glu 308 | - | expression tag |
| Glu 309 | - | expression tag |
| Lys 310 | - | expression tag |
| Arg 468 | Ser 492 | engineered mutation |
| His 515 | - | expression tag |
| His 516 | - | expression tag |
| His 517 | - | expression tag |
| His 518 | - | expression tag |
| His 519 | - | expression tag |
| His 520 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 281828.4 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 4205.9 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1548.5 | |
| All* | Total formula weight | 287582.7 |
