6B3J

3.3 angstrom phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Glucagon-like peptide 1 receptor	polymer	491	56748.4	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (Human)	GLP-1R
2	B (P)	Exendin-P5	polymer	40	4228.7	1		synthetic construct
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
6	F (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama (llama)

Sequence modifications

R: 24 - 463 (UniProt: P43220)

PDB	External Database	Details
Met -8	-	initiating methionine
Lys -7	-	expression tag
Thr -6	-	expression tag
Ile -5	-	expression tag
Ile -4	-	expression tag
Ala -3	-	expression tag
Leu -2	-	expression tag
Ser -1	-	expression tag
Tyr 0	-	expression tag
Ile 1	-	expression tag
Phe 2	-	expression tag
Cys 3	-	expression tag
Leu 4	-	expression tag
Val 5	-	expression tag
Phe 6	-	expression tag
Ala 7	-	expression tag
Asp 8	-	expression tag
Tyr 9	-	expression tag
Lys 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Leu 15	-	expression tag
Glu 16	-	expression tag
Val 17	-	expression tag
Leu 18	-	expression tag
Phe 19	-	expression tag
Gln 20	-	expression tag
Gly 21	-	expression tag
Pro 22	-	expression tag
Ala 23	-	expression tag
Phe 260	Leu 260	variant
Pro 464	-	expression tag
Ala 465	-	expression tag
Gly 466	-	expression tag
Leu 467	-	expression tag
Glu 468	-	expression tag
Val 469	-	expression tag
Leu 470	-	expression tag
Phe 471	-	expression tag
Gln 472	-	expression tag
Gly 473	-	expression tag
Pro 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	168196.5
All*	Total formula weight	168196.5

*Water molecules are not included.