6ASO

Structure of yeast U6 snRNP with 3'-phosphate terminated U6 RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	U4/U6 snRNA-associated-splicing factor PRP24	polymer	424	49049.6	1	UniProt (P49960) Pfam (PF00076) Pfam (PF16842) Pfam (PF05391) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U4/U6 snRNP protein
2	B	U6 snRNA-associated Sm-like protein LSm2	polymer	95	11177.9	1	UniProt (P38203) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Small nuclear ribonucleoprotein D homolog SNP3
3	C	U6 snRNA-associated Sm-like protein LSm3	polymer	97	11081.4	1	UniProt (P57743) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	SmX4 protein
4	D	U6 snRNA-associated Sm-like protein LSm4	polymer	93	10627.1	1	UniProt (P40070) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
5	E	U6 snRNA-associated Sm-like protein LSm5	polymer	93	10433.0	1	UniProt (P40089) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
6	F	U6 snRNA-associated Sm-like protein LSm6	polymer	88	9550.7	1	UniProt (A6ZYX7) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
7	G	U6 snRNA-associated Sm-like protein LSm7	polymer	115	13027.0	1	UniProt (P53905) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
8	H	U6 snRNA-associated Sm-like protein LSm8	polymer	115	13232.3	1	UniProt (P47093) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)
9	I	Saccharomyces cerevisiae strain HB_S_GIMBLETTROAD_9 chromosome XII sequence	polymer	84	27017.9	1	GenBank (1039023481)	Saccharomyces cerevisiae
10	A, I	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
11	I	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
12	I	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
13		water	water		18.0	134	Chemie (HOH)

Sequence modifications

A: 28 - 444 (UniProt: P49960)

PDB	External Database	Details
Met 27	-	initiating methionine
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag

C: 1 - 89 (UniProt: P57743)

PDB	External Database	Details
Trp 90	-	expression tag
Ser 91	-	expression tag
His 92	-	expression tag
Pro 93	-	expression tag
Gln 94	-	expression tag
Phe 95	-	expression tag
Glu 96	-	expression tag
Lys 97	-	expression tag

F: 1 - 86 (UniProt: A6ZYX7)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

H: 1 - 109 (UniProt: P47093)

PDB	External Database	Details
His 110	-	expression tag
His 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag

I: 30 - 113 (GenBank: 1039023481)

PDB	External Database	Details
G 62	A 366024	conflict
G 79	A 366041	conflict

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	155196.9
Non-Polymers*	Number of molecules	5
	Total formula weight	181.7
All*	Total formula weight	155378.6

*Water molecules are not included.