6ARU

Structure of Cetuximab Fab mutant in complex with EGFR extracellular domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Epidermal growth factor receptor	polymer	622	69015.6	1	UniProt (P00533) Pfam (PF01030) Pfam (PF00757) Pfam (PF14843)	Homo sapiens (Human)	Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
2	B (B)	Cetuximab mutant light chain,Uncharacterized protein	polymer	214	23504.9	1	UniProt (Q8TCD0)	Homo sapiens (Human)
3	C (C)	Cetuximab mutant heavy chain Fab fragment,Immunoglobulin gamma-1 heavy chain	polymer	222	23829.6	1	UniProt (P0DOX5)	Homo sapiens (Human)
4	D (D)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G96786JM)
5	E (E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	F, G, H (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A: 1 - 616 (UniProt: P00533)

PDB	External Database	Details
Lys 516	Asn 540	conflict
Arg 610	Glu 634	conflict
His 617	-	expression tag
His 618	-	expression tag
His 619	-	expression tag
His 620	-	expression tag
His 621	-	expression tag
His 622	-	expression tag

B: 1 - 106 (PDB: 6ARU)
B: 107 - 214 (UniProt: Q8TCD0)
C: 1 - 119 (PDB: 6ARU)
C: 120 - 222 (UniProt: P0DOX5)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	116350.1
Branched	Number of molecules	2
	Total formula weight	1821.6
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	118835.4

*Water molecules are not included.