6ARU
Structure of Cetuximab Fab mutant in complex with EGFR extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.495, 70.444, 149.079 |
| Unit cell angles | 90.00, 100.95, 90.00 |
Refinement procedure
| Resolution | 40.108 - 3.200 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yy9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.322 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 26340 | 4724 |
| <I/σ(I)> | 7.6 | 1.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.988 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 150 mM ammonium sulfate, 16.5% PEG3350, 10 mM cadmium chloride, 100 mM imidazole, 5% glycerol |
