6AN2
STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING D4TTP AT PH 7.5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, C) | HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT | polymer | 556 | 64038.4 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
| 2 | B, F (B, D) | HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT | polymer | 444 | 51928.6 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
| 3 | C, G (T, E) | DNA TEMPLATE (5'- D(*AP*TP*GP*AP*AP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3') | polymer | 27 | 8376.4 | 2 | synthetic construct | ||
| 4 | D, H (P, F) | DNA PRIMER (5'- D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*GP)-3') | polymer | 21 | 6400.1 | 2 | synthetic construct | ||
| 5 | I, J, T, U (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 6 | K, V (A, C) | 2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-TRIPHOSPHATE | non-polymer | 464.2 | 2 | Chemie (D4T) | |||
| 7 | AA, BA, L, M, N... (D, B, C) | GLYCEROL | non-polymer | 92.1 | 14 | Chemie (GOL) | |||
| 8 | CA, DA, EA, FA, GA... (A, B, T, P, C...) | water | water | 18.0 | 615 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 554 (UniProt: P03366)
B, D: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Val 0 | - | expression tag |
| Cys 63 | Ile 662 | engineered mutation |
| Ser 280 | Cys 879 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Ala -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| Ala -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 280 | Cys 879 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 261487.0 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 2314.8 | |
| All* | Total formula weight | 263801.9 |
