6AMC

Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB4D11

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Tryptophan synthase beta chain 1	polymer	396	43694.8	4	UniProt (Q8U093) Pfam (PF00291) In PDB	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
2	A, B, C, D	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
3		water	water		18.0	263	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 388 (UniProt: Q8U093)

PDB	External Database	Details
Gly 17	Glu 17	engineered mutation
Val 68	Ile 68	engineered mutation
Ser 274	Phe 274	engineered mutation
Ser 292	Thr 292	engineered mutation
Ala 321	Thr 321	engineered mutation
Leu 389	-	expression tag
Glu 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	174779.3
Non-Polymers*	Number of molecules	4
	Total formula weight	92.0
All*	Total formula weight	174871.2

*Water molecules are not included.