6AMC
Engineered tryptophan synthase b-subunit from Pyrococcus furiosus, PfTrpB4D11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.438, 110.729, 160.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.930 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dvz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rpim | 0.023 | 0.547 |
| Number of reflections | 114421 | 5601 |
| <I/σ(I)> | 15.4 | 1.3 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 0.998 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.85 | 298 | 15-25% PEG3350 and 0.1 M Na HEPES pH 7.85 |
