6AK3

Crystal structure of the human prostaglandin E receptor EP3 bound to prostaglandin E2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Prostaglandin E2 receptor EP3 subtype,Soluble cytochrome b562	polymer	418	46044.7	2	UniProt (P43115) UniProt (P0ABE7) Pfam (PF00001) Pfam (PF07361)	Homo sapiens (Human)	PGE2 receptor EP3 subtype,PGE2-R,Prostanoid EP3 receptor,Cytochrome b-562
2	C, E (B, A)	(Z)-7-[(1R,2R,3R)-3-hydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]-5-oxo-cyclopentyl]hept-5-enoic acid	non-polymer		352.5	2	Chemie (P2E)
3	D, F (B, A)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	2	Chemie (POV)

Sequence modifications

B, A: 43 - 359 (UniProt: P43115)

PDB	External Database	Details
Gly 39	-	expression tag
Pro 40	-	expression tag
Thr 41	-	expression tag
Ser 42	-	expression tag
Ile 173	Ala 173	engineered mutation
Ser 185	Val 185	engineered mutation
Gln 217	Asn 217	engineered mutation
Asp 258	Ser 258	engineered mutation
Leu 289	Cys 289	engineered mutation
Gln 308	Asn 308	engineered mutation
Leu 360	-	linker
Glu 361	-	linker
Leu 362	-	linker
Glu 363	-	linker
Val 364	-	linker
Leu 365	-	linker
Phe 366	-	linker
Gln 367	-	linker

B, A: 1001 - 1040 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 1007	Met 29	engineered mutation

B, A: 1063 - 1105 (UniProt: P0ABE7)

PDB	External Database	Details
Gly 1041	-	linker
Ser 1042	-	linker
Gly 1043	-	linker
Ser 1044	-	linker
Gly 1045	-	linker
Ile 1098	Arg 120	engineered mutation
Ile 1102	His 124	engineered mutation
Gly 1106	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92089.4
Non-Polymers*	Number of molecules	4
	Total formula weight	2225.1
All*	Total formula weight	94314.5

*Water molecules are not included.