6AH7

D45W/H226G mutant of marine bacterial prolidase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Xaa-Pro dipeptidase	polymer	448	51629.2	4	UniProt (A0A1I7CHQ2)	Pseudoalteromonas lipolytica	X-Pro dipeptidase,Imidodipeptidase,Proline dipeptidase,Prolidase
2	E, F, I, J, K... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	G (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	H (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	O, P, Q, R (A, B, C, D)	water	water		18.0	565	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 440 (UniProt: A0A1I7CHQ2)

PDB	External Database	Details
Trp 45	Asp 45	engineered mutation
Gly 226	His 226	engineered mutation
Leu 441	-	expression tag
Asp 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	206516.7
Non-Polymers*	Number of molecules	10
	Total formula weight	558.6
All*	Total formula weight	207075.2

*Water molecules are not included.