6AH7
D45W/H226G mutant of marine bacterial prolidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2018-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | H 3 2 |
Unit cell lengths | 183.632, 183.632, 371.306 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.290 - 2.380 |
Rwork | 0.233 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zwo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.641 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.290 | 2.500 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.198 | 1.498 |
Number of reflections | 94764 | 14041 |
<I/σ(I)> | 12.5 | 2.4 |
Completeness [%] | 97.8 | 100 |
Redundancy | 19.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2 M Ammonium sulfate, 5% PEG400, 100 mM MES pH6.5 |