6AFO

Crystal structure of class A b-lactamase, PenL, variant Asn136Asp, from Burkholderia thailandensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-lactamase	polymer	268	28678.4	2	UniProt (A0A2Z4SUB5) Pfam (PF00144) Pfam (PF13354) UniProt (by SIFTS) (Q2T5A3)	Burkholderia thailandensis
2	C, D (A, B)	water	water		18.0	723	Chemie (HOH)

Sequence modifications

A, B: 26 - 291 (UniProt: A0A2Z4SUB5)

PDB	External Database	Details
Gly 22	-	expression tag
Ser 23	-	expression tag
His 24	-	expression tag
Met 25	-	expression tag
Asp 136	Asn 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57356.9
All*	Total formula weight	57356.9

*Water molecules are not included.