6AFN
Crystal structure of class A b-lactamase, PenL, variant Cys69Tyr, from Burkholderia thailandensis, in complex with ceftazidime-like boronic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-lactamase | polymer | 268 | 28737.5 | 1 | UniProt (A0A2Z4SUB5) Pfam (PF00144) Pfam (PF13354) UniProt (by SIFTS) (Q2T5A3) | Burkholderia thailandensis | |
| 2 | B (A) | PINACOL[[2-AMINO-ALPHA-(1-CARBOXY-1-METHYLETHOXYIMINO)-4-THIAZOLEACETYL]AMINO]METHANEBORONATE | non-polymer | 330.1 | 1 | Chemie (CB4) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | D (A) | water | water | 18.0 | 369 | Chemie (HOH) |
Sequence modifications
A: 26 - 291 (UniProt: A0A2Z4SUB5)
| PDB | External Database | Details |
|---|---|---|
| Gly 22 | - | expression tag |
| Ser 23 | - | expression tag |
| His 24 | - | expression tag |
| Met 25 | - | expression tag |
| Tyr 69 | Cys 74 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28737.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 422.2 | |
| All* | Total formula weight | 29159.7 |
