6AE8

Structure insight into histone chaperone Chz1-mediated H2A.Z recognition and replacement

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Histone H2B.1,Histone H2A.Z	polymer	193	21205.4	2	UniProt (P02293) UniProt (Q12692) Pfam (PF00125) Pfam (PF16211)	Saccharomyces cerevisiae S288c (Baker's yeast)
2	B, D (C, D)	Histone H2A.Z-specific chaperone CHZ1	polymer	120	13511.2	2	UniProt (P40019) Pfam (PF09649)	Saccharomyces cerevisiae S288c (Baker's yeast)
3	E, F (A, B)	BICINE	non-polymer		163.2	2	Chemie (BCN)
4	G, H, I, J (A, C, B, D)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

A, B: 1 - 95 (UniProt: P02293)

PDB	External Database	Details
Met 0	-	initiating methionine

A, B: 96 - 192 (UniProt: Q12692)
C, D: 1 - 90 (UniProt: P40019)

PDB	External Database	Details
Met -29	-	initiating methionine
Gly -28	-	expression tag
Ser -27	-	expression tag
Ser -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Tyr -19	-	expression tag
Pro -18	-	expression tag
Tyr -17	-	expression tag
Asp -16	-	expression tag
Val -15	-	expression tag
Pro -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Ala -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69433.2
Non-Polymers*	Number of molecules	2
	Total formula weight	326.3
All*	Total formula weight	69759.6

*Water molecules are not included.