6AE8
Structure insight into histone chaperone Chz1-mediated H2A.Z recognition and replacement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2016-04-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.082, 46.210, 56.662 |
| Unit cell angles | 72.37, 70.92, 70.60 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.18321 |
| Rwork | 0.181 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5chl |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rpim | 0.048 | 0.500 |
| Number of reflections | 42986 | 4146 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 96.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | Bicine,1,4-Dioxane,PEG 20000 |
