6AB2
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with oClZLys
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysine--tRNA ligase | polymer | 274 | 31347.0 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | Methanosarcina mazei JCM 9314 (Methanosarcina frisia) | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | B, C, D (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 3 | E (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 4 | F, G (A) | (2S)-2-azanyl-6-[(2-chlorophenyl)methoxycarbonylamino]hexanoic acid | non-polymer | 314.8 | 2 | Chemie (9V6) | |||
| 5 | H, I (A) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | J, K, L (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 7 | M (A) | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Gly 181 | - | expression tag |
| Ser 182 | - | expression tag |
| His 183 | - | expression tag |
| Met 184 | - | expression tag |
| Ala 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
| Gly 444 | Glu 444 | see sequence details |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31347.0 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 1474.0 | |
| All* | Total formula weight | 32821.0 |
