6AAO
Crystal structure of Methanosarcina mazei PylRS(Y306A/Y384F) complexed with TCO*Lys
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pyrrolysine--tRNA ligase | polymer | 274 | 31347.0 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | Methanosarcina mazei JCM 9314 (Methanosarcina frisia) | Pyrrolysine--tRNA(Pyl) ligase,Pyrrolysyl-tRNA synthetase,PylRS |
| 2 | B, C, D (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 3 | E (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 4 | F (A) | N6-({[(1S,2E)-cyclooct-2-en-1-yl]oxy}carbonyl)-L-lysine | non-polymer | 298.4 | 1 | Chemie (9U0) | |||
| 5 | G, H (A) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | I (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | J (A) | water | water | 18.0 | 294 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Gly 181 | - | expression tag |
| Ser 182 | - | expression tag |
| His 183 | - | expression tag |
| Met 184 | - | expression tag |
| Ala 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
| Gly 444 | Glu 444 | see sequence details |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 31347.0 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1062.8 | |
| All* | Total formula weight | 32409.8 |
