6A8I

Crystal structure of endo-arabinanase ABN-TS D147N mutant in complex with arabinohexaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	endo-alpha-(1->5)-L-arabinanase	polymer	321	36557.7	2	UniProt (Q93HT9) Pfam (PF04616)	Geobacillus thermodenitrificans	arabinanase-TS,ABN,Endo-1,5-alpha-L-arabinanase
2	C, D (C, D)	alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose-(1-5)-alpha-L-arabinofuranose	branched		810.7	2	In PDB GlyTouCan (G40506IV)
3	E, F (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	G, H (A, B)	water	water		18.0	461	Chemie (HOH)

Sequence modifications

A, B: 1 - 313 (UniProt: Q93HT9)

PDB	External Database	Details
Asn 147	Asp 147	engineered mutation
Leu 314	-	expression tag
Glu 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73115.5
Branched	Number of molecules	2
	Total formula weight	1621.4
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	74817.0

*Water molecules are not included.