6A8I
Crystal structure of endo-arabinanase ABN-TS D147N mutant in complex with arabinohexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.809, 92.260, 78.673 |
| Unit cell angles | 90.00, 91.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18358 |
| Rwork | 0.181 |
| R-free | 0.23286 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wl7 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.977 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.055 | 0.324 |
| Number of reflections | 51538 | 2522 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 16% PEG 8000, 0.2M magnesium chloride, 0.1M MES pH 5.5 |
