6A0F

Crystal structure of human protein N-terminal asparagine amidohydrolase (NTAN1) C75S mutant with Asn-Phe-Ala-Ala-Arg peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Protein N-terminal asparagine amidohydrolase	polymer	318	35778.2	2	UniProt (Q96AB6) Pfam (PF14736)	Homo sapiens (Human)	Protein NH2-terminal asparagine amidohydrolase,PNAA,Protein NH2-terminal asparagine deamidase,Protein NTN-amidase
2	C, D (C, D)	5-mer peptide Asn-Phe-Ala-Ala-Arg	polymer	5	578.6	2		synthetic construct
3	E, F, G, H, I... (A, B)	GLYCEROL	non-polymer		92.1	13	Chemie (GOL)
4	N, O, P, Q, R... (A, B)	PHOSPHATE ION	non-polymer		95.0	8	Chemie (PO4)
5	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	218	Chemie (HOH)

Sequence modifications

A, B: 1 - 310 (UniProt: Q96AB6)

PDB	External Database	Details
Ser 75	Cys 75	engineered mutation
Leu 311	-	expression tag
Glu 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72713.7
Non-Polymers*	Number of molecules	21
	Total formula weight	1957.0
All*	Total formula weight	74670.7

*Water molecules are not included.