6A0F

Crystal structure of human protein N-terminal asparagine amidohydrolase (NTAN1) C75S mutant with Asn-Phe-Ala-Ala-Arg peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-17
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	84.045, 85.749, 88.297
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.757 - 2.384
R-factor	0.1982
Rwork	0.196
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a0i
RMSD bond length	0.002
RMSD bond angle	0.464
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rpim	0.035	0.190
Number of reflections	25940	1246
<I/σ(I)>	18.41
Completeness [%]	99.9
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	295	PEG 3350, potassium phosphate monobasic