5ZXM

Crystal Structure of GyraseB N-terminal at 1.93A Resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA gyrase subunit B	polymer	400	44241.7	2	UniProt (A0A3R0U328) Pfam (PF02518) Pfam (PF00204)	Salmonella enterica I
2	C, H (A, B)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
3	D, I (A, B)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E, F, J (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
5	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	K, L (B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
7	M, N (A, B)	water	water		18.0	560	Chemie (HOH)

Sequence modifications

A, B: 1 - 394 (UniProt: A0A3R0U328)

PDB	External Database	Details
Ala 40	Val 40	engineered mutation
Ser 85	Glu 85	engineered mutation
Lys 173	His 173	engineered mutation
Ser 174	Glu 174	engineered mutation
His 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88483.5
Non-Polymers*	Number of molecules	10
	Total formula weight	1573.4
All*	Total formula weight	90056.9

*Water molecules are not included.