5ZNZ

Structure of mDR3 DD with MBP tag mutant-I387V

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Maltose-binding periplasmic protein,Tumor necrosis factor receptor superfamily, member 25	polymer	464	51356.3	4	UniProt (P0AEX9) UniProt (B1AWN9) Pfam (PF01547) Pfam (PF00531) In PDB	Escherichia coli (strain K12)	MBP tag, MBP,MMBP,Maltodextrin-binding protein,Tumor necrosis factor receptor superfamily,member 25,isoform CRA_b
2	D, A, B, C	SULFATE ION	non-polymer		96.1	26	Chemie (SO4)
3		water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 367 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	expression tag
Ala 83	Asp 108	engineered mutation
Ala 84	Lys 109	engineered mutation
Ala 173	Glu 198	engineered mutation
Ala 174	Asn 199	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	Lys 388	engineered mutation
Ala 364	Asp 389	engineered mutation
Asn 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
Arg 371	-	linker
Ala 372	-	linker
Ala 373	-	linker
Ala 374	-	linker

A, B, C, D: 375 - 456 (UniProt: B1AWN9)

PDB	External Database	Details
Val 434	Ile 387	engineered mutation
Leu 457	-	expression tag
Glu 458	-	expression tag
His 459	-	expression tag
His 460	-	expression tag
His 461	-	expression tag
His 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	205425.0
Non-Polymers*	Number of molecules	26
	Total formula weight	2497.6
All*	Total formula weight	207922.6

*Water molecules are not included.