5ZNZ
Structure of mDR3 DD with MBP tag mutant-I387V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2018-01-25 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.400, 144.870, 177.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.527 - 2.550 |
Rwork | 0.234 |
R-free | 0.27380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omp |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 75446 | |
<I/σ(I)> | 17.51 | |
Completeness [%] | 99.5 | |
Redundancy | 6.53 | |
CC(1/2) | 0.999 | 0.806 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2.5 M ammonium sulfate, 0.1 M MES 5.5 |