5ZLZ

Structure of tPA and PAI-1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (I)	Plasminogen activator inhibitor 1	polymer	374	42212.4	1	UniProt (P05121) Pfam (PF00079)	Homo sapiens (Human)	PAI-1,Endothelial plasminogen activator inhibitor,Serpin E1
2	B (E)	Tissue-type plasminogen activator	polymer	251	28008.7	1	UniProt (P00750) Pfam (PF00089)	Homo sapiens (Human)	tPA
3	C, D (E)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)

Sequence modifications

I: 6 - 379 (UniProt: P05121)

PDB	External Database	Details
His 150	Asn 173	engineered mutation
Thr 154	Lys 177	engineered mutation
Leu 319	Gln 342	engineered mutation
Ile 354	Met 377	engineered mutation

E: 16 - 243 (UniProt: P00750)

PDB	External Database	Details
Ala 122	Cys 430	engineered mutation
Glu 150	Phe 458	engineered mutation
Gln 173	Asn 483	engineered mutation
Ala 195	Ser 513	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70221.0
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	70405.2

*Water molecules are not included.