5ZKB

Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with AF-DX 384

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muscarinic acetylcholine receptor M2,Apo-cytochrome b562,Muscarinic acetylcholine receptor M2	polymer	421	47293.2	1	UniProt (P08172) Pfam (PF00001) Pfam (PF07361) UniProt (by SIFTS) (P0ABE7)	Homo sapiens (Human)
2	B (A)	N-[2-[(2S)-2-[(dipropylamino)methyl]piperidin-1-yl]ethyl]-6-oxidanylidene-5H-pyrido[2,3-b][1,4]benzodiazepine-11-carboxamide	non-polymer		478.6	1	Chemie (82F)

Sequence modifications

A: 10 - 217 (UniProt: P08172)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Ser 4	-	expression tag
Thr 5	-	expression tag
Asp 6	-	expression tag
Ser 7	-	expression tag
Ser 8	-	expression tag
Asp 9	-	expression tag
Arg 110	Ser 110	engineered mutation

A: 1001 - 1106 (PDB: 5ZKB)
A: 377 - 466 (UniProt: P08172)

PDB	External Database	Details
Leu 467	-	expression tag
Glu 468	-	expression tag
Val 469	-	expression tag
Leu 470	-	expression tag
Phe 471	-	expression tag
Gln 472	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47293.2
Non-Polymers*	Number of molecules	1
	Total formula weight	478.6
All*	Total formula weight	47771.8

*Water molecules are not included.