5ZKB

Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with AF-DX 384

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004930	molecular_function	G protein-coupled receptor activity
A	0005506	molecular_function	iron ion binding
A	0005886	cellular_component	plasma membrane
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0009055	molecular_function	electron transfer activity
A	0016020	cellular_component	membrane
A	0016907	molecular_function	G protein-coupled acetylcholine receptor activity
A	0020037	molecular_function	heme binding
A	0022900	biological_process	electron transport chain
A	0042597	cellular_component	periplasmic space
A	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue 82F A 1201

Chain	Residue
A	TRP99
A	PHE195
A	TRP400
A	TYR403
A	ASN404
A	TYR426
A	CYS429
A	TYR430
A	LEU100
A	ASP103
A	TYR104
A	SER107
A	ASN108
A	THR190
A	ALA191
A	ALA194

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	25
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	33
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	31
Details	Topological domain: {"description":"Extracellular","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	26
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	21
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	24
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	22
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"PubMed","id":"36690613","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"7T8X","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T90","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T94","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7T96","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	3
Details	Motif: {"description":"P-X-P-P motif","evidences":[{"source":"PubMed","id":"37209686","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	18
Details	Compositional bias: {"description":"Polar residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"38175886","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8J8R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"38175886","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8J8R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8J97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JAF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"38175886","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8J8R","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8J97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JAF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"38175886","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8J97","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8JAF","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"38175886","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8J8R","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	2
Details	Binding site: {"description":"axial binding residue"}

Chain

Residue

Details

---