5ZKA

Crystal structure of N-acetylneuraminate lyase from Fusobacterium nucleatum complexed with Pyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	N-acetylneuraminate lyase	polymer	307	34983.4	1	UniProt (Q8RDN6) Pfam (PF00701)	Fusobacterium nucleatum subsp. nucleatum ATCC 25586	Neu5Ac lyase,N-acetylneuraminate pyruvate-lyase,N-acetylneuraminic acid aldolase,Sialate lyase,Sialic acid aldolase,Sialic acid lyase
2	B (B)	N-acetylneuraminate lyase	polymer	296	33538.8	1	UniProt (Q8RDN6) Pfam (PF00701)	Fusobacterium nucleatum subsp. nucleatum ATCC 25586	Neu5Ac lyase,N-acetylneuraminate pyruvate-lyase,N-acetylneuraminic acid aldolase,Sialate lyase,Sialic acid aldolase,Sialic acid lyase
3	C, D, E, F, G (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
4	H (B)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	I, J (A, B)	water	water		18.0	166	Chemie (HOH)

Sequence modifications

A: 1 - 290 (UniProt: Q8RDN6)

PDB	External Database	Details
Met -16	-	initiating methionine
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ile -9	-	expression tag
Thr -8	-	expression tag
Ser -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Lys -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Phe 0	-	expression tag

B: 1 - 289 (UniProt: Q8RDN6)

PDB	External Database	Details
Leu -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Lys -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68522.2
Non-Polymers*	Number of molecules	6
	Total formula weight	460.5
All*	Total formula weight	68982.7

*Water molecules are not included.