5ZK3

Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with QNB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Muscarinic acetylcholine receptor M2,Apo-cytochrome b562,Muscarinic acetylcholine receptor M2	polymer	421	47293.2	1	UniProt (P08172) Pfam (PF00001) Pfam (PF07361) UniProt (by SIFTS) (P0ABE7) In PDB	Homo sapiens (Human)
2	A	(3R)-1-azabicyclo[2.2.2]oct-3-yl hydroxy(diphenyl)acetate	non-polymer		337.4	1	Chemie (QNB)
3		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 10 - 217 (UniProt: P08172)

PDB	External Database	Details
Gly -1	-	expression tag
Pro 0	-	expression tag
Met 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Ser 4	-	expression tag
Thr 5	-	expression tag
Asp 6	-	expression tag
Ser 7	-	expression tag
Ser 8	-	expression tag
Asp 9	-	expression tag
Arg 110	Ser 110	engineered mutation

A: 1001 - 1106 (PDB: 5ZK3)
A: 377 - 466 (UniProt: P08172)

PDB	External Database	Details
Leu 467	-	expression tag
Glu 468	-	expression tag
Val 469	-	expression tag
Leu 470	-	expression tag
Phe 471	-	expression tag
Gln 472	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47293.2
Non-Polymers*	Number of molecules	1
	Total formula weight	337.4
All*	Total formula weight	47630.6

*Water molecules are not included.