5ZHP

M3 muscarinic acetylcholine receptor in complex with a selective antagonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Muscarinic acetylcholine receptor M3,Endolysin,Endolysin,Muscarinic acetylcholine receptor M3	polymer	418	47928.1	2	UniProt (P08483) UniProt (D9IEF7) Pfam (PF00001) Pfam (PF00959) UniProt (by SIFTS) (P00720)	Rattus norvegicus (Rat)	Lysis protein,Lysozyme,Muramidase,Lysis protein,Lysozyme,Muramidase
2	C (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	D (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
4	E, F (A, B)	(1R,2R,4S,5S,7s)-7-({[4-fluoro-2-(thiophen-2-yl)phenyl]carbamoyl}oxy)-9,9-dimethyl-3-oxa-9-azatricyclo[3.3.1.0~2,4~]nonan-9-ium	non-polymer		389.5	2	Chemie (9EC)
5	G (B)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
6	H (B)	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)

Sequence modifications

A, B: 57 - 259 (UniProt: P08483)

PDB	External Database	Details
Gly 56	-	expression tag
Arg 154	Ser 154	engineered mutation

A, B: 1001 - 1012 (UniProt: D9IEF7)
A, B: 1018 - 1118 (UniProt: D9IEF7)

PDB	External Database	Details
Gly 1013	-	linker
Gly 1014	-	linker
Ser 1015	-	linker
Gly 1016	-	linker
Gly 1017	-	linker
Ala 1054	Cys 97	engineered mutation

A, B: 482 - 569 (UniProt: P08483)

PDB	External Database	Details
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95856.3
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1447.6
All*	Total formula weight	97646.2

*Water molecules are not included.