5ZEA

Crystal structure of the nucleotide-free mutant A3B3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, G, H... (A, B, C, G, H...)	V-type sodium ATPase catalytic subunit A	polymer	594	65771.2	6	UniProt (Q08636)	Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)	Na(+)-translocating ATPase subunit A,V-type sodium pump catalytic subunit A
2	D, E, F, J, K... (D, E, F, J, K...)	V-type sodium ATPase subunit B	polymer	462	51455.6	6	UniProt (Q08637)	Enterococcus hirae (strain ATCC 9790 / DSM 20160 / JCM 8729 / LMG 6399 / NBRC 3181 / NCIMB 6459 / NCDO 1258)	Na(+)-translocating ATPase subunit B,V-type sodium pump subunit B
3	AA, BA, CA, M, N... (G, H, K, A, C...)	GLYCEROL	non-polymer		92.1	17	Chemie (GOL)
4	DA, EA, FA, GA, HA... (A, B, C, D, E...)	water	water		18.0	100	Chemie (HOH)

Sequence modifications

A, B, C, G, H, I: 1 - 587 (UniProt: Q08636)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

D, E, F, J, K, L: 1 - 455 (UniProt: Q08637)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Tyr 65	Leu 65	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	703360.7
Non-Polymers*	Number of molecules	17
	Total formula weight	1565.6
All*	Total formula weight	704926.3

*Water molecules are not included.