5ZD4

Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (C, D, A, B)	Maltose-binding periplasmic protein,Protein BRASSINAZOLE-RESISTANT 1	polymer	453	49819.4	4	UniProt (P0AEY0) UniProt (Q8S307) Pfam (PF01547) Pfam (PF05687)	Escherichia coli O157:H7	MBP,MMBP,Maltodextrin-binding protein,Protein BIN2 SUBSTRATE 2
2	C, D, G, H (G, H, E, F)	DNA (5'-D(*TP*TP*CP*AP*CP*AP*CP*GP*TP*GP*TP*GP*AP*AP*A)-3')	polymer	15	4593.0	4		synthetic construct
3	I, J, K, L (I, J, K, L)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	4	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4	M, N, O, P, Q... (C, D, A, B)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
5	AA, T, U, V, W... (F, C, D, G, H...)	water	water		18.0	283	Chemie (HOH)

Sequence modifications

C, D, A, B: -367 - -2 (UniProt: P0AEY0)

PDB	External Database	Details
Met -368	-	expression tag
Ala -286	Asp 108	engineered mutation
Ala -285	Lys 109	engineered mutation
Ala -196	Glu 198	engineered mutation
Ala -195	Asn 199	engineered mutation
Ala -129	Lys 265	engineered mutation
Ala -9	Glu 385	engineered mutation
Ala -6	Lys 388	engineered mutation
Ala -5	Asp 389	engineered mutation
Asn -1	-	linker
Ala 0	-	linker

C, D, A, B: 21 - 104 (UniProt: Q8S307)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	217649.8
Branched	Number of molecules	4
	Total formula weight	1369.2
Non-Polymers*	Number of molecules	7
	Total formula weight	434.5
All*	Total formula weight	219453.5

*Water molecules are not included.