5ZD4
Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-04-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.701, 92.603, 111.967 |
Unit cell angles | 90.00, 100.42, 90.00 |
Refinement procedure
Resolution | 43.598 - 2.170 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5iqz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.786 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.330 | 2.210 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmeas | 0.061 | 0.578 |
Rpim | 0.033 | 0.320 |
Number of reflections | 109100 | 5420 |
<I/σ(I)> | 21.2 | 3.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50mM sodium cacodylate (pH 6.5), 200mM potassium chloride, 10% (w/v) polyethylene glycol (PEG) 4000 |