5ZCW

Structure of the Methanosarcina mazei class II CPD-photolyase in complex with intact, phosphodiester linked, CPD-lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Deoxyribodipyrimidine photolyase	polymer	482	55123.5	2	UniProt (A0A0F8I5V2) Pfam (PF00875) UniProt (by SIFTS) (Q8PYK9) In PDB	Methanosarcina mazei (Methanosarcina frisia)
2	C, E	5'-D(*AP*TP*CP*GP*GP*CP*(TTD)P*CP*GP*CP*GP*CP*AP*A)-3'	polymer	14	4570.0	2		synthetic construct
3	D, F	5'-D(*TP*GP*CP*GP*CP*GP*AP*AP*GP*CP*CP*GP*AP*T)-3'	polymer	14	4305.8	2		synthetic construct
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
6	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
7		water	water		18.0	338	Chemie (HOH)

Sequence modifications

A, B: 3 - 464 (UniProt: A0A0F8I5V2)

PDB	External Database	Details
Met -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Pro -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
His 2	-	expression tag
Thr 377	Met 375	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	127998.5
Non-Polymers*	Number of molecules	4
	Total formula weight	1726.2
All*	Total formula weight	129724.7

*Water molecules are not included.